B cell surface antigen CD40 Protein, Bp50 Protein, CD40 Protein, CD40L receptor Protein, CDW40 Protein, p50 Protein, TNFRSF5 Protein
Catalogue No.:
P8404
Uniprot ID:
P25942
Host:
CHO cells
Expression Region:
21-193
Mol. Weight:
32 kDa
Tags:
C-terminal His Tag
SPECIFICATIONS
Product Name
Recombinant Human CD40
Catalogue No.
P8404
Species
Human
Host
CHO cells
Expression Region
21-193
Mol. Weight
32 kDa
Tags
C-terminal His Tag
Purity
Greater than 95% as determined by SDS-PAGE
Application
Western Blot, ELISA
Activity
Measured by its binding ability in a functional ELISA.Immobilized recombinant human CD40 at 1 μg/ml (100 μl/well) can bind biotinylated human CD40L with a linear range of 25-600 ng/ml.
Formulation
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose and 0.06% proclin, pH7.4
Reconstitution
Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex
Storage
The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.
Alternative Names
B cell surface antigen CD40 Protein, Bp50 Protein, CD40 Protein, CD40L receptor Protein, CDW40 Protein, p50 Protein, TNFRSF5 Protein